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CapitalBio Corporation microarray experiment program
The gene expression profiles and interaction network of top 50 GO terms in preB cells with BP9 treatment. (a) The gene expression profiles of BP9-treated WEHI-231 cells. WEHI-231 cells were treated with 0.01 mg/mL BP9 for 4 h, and the expressions of the differentially regulated genes were detected with <t>microarray;</t> the bar indicated that up- (red) and downregulated (green) genes were higher and lower 2-fold than that of control without BP9 treatment. (b) Gene expression validation. Seven differentially expressed genes obtained from WEHI-231 cells after BP9 treatment were validated with qRT-PCR. (c) The top 50 GO terms selected for finding the vital biological functions in preB cells with BP9 treatment. Blue, green, and orange bars indicated the biological processes, cellular component, and molecular functions among the top 50 GO terms, respectively. (d) Interaction network of top 50 GO terms in preB cells with BP9 treatment. The GO terms were generated by analyzing all the differentially expressed genes in WEHI-231 cells with BP9 treatment.
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Image Search Results


The gene expression profiles and interaction network of top 50 GO terms in preB cells with BP9 treatment. (a) The gene expression profiles of BP9-treated WEHI-231 cells. WEHI-231 cells were treated with 0.01 mg/mL BP9 for 4 h, and the expressions of the differentially regulated genes were detected with microarray; the bar indicated that up- (red) and downregulated (green) genes were higher and lower 2-fold than that of control without BP9 treatment. (b) Gene expression validation. Seven differentially expressed genes obtained from WEHI-231 cells after BP9 treatment were validated with qRT-PCR. (c) The top 50 GO terms selected for finding the vital biological functions in preB cells with BP9 treatment. Blue, green, and orange bars indicated the biological processes, cellular component, and molecular functions among the top 50 GO terms, respectively. (d) Interaction network of top 50 GO terms in preB cells with BP9 treatment. The GO terms were generated by analyzing all the differentially expressed genes in WEHI-231 cells with BP9 treatment.

Journal: Journal of Immunology Research

Article Title: The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy

doi: 10.1155/2019/1574383

Figure Lengend Snippet: The gene expression profiles and interaction network of top 50 GO terms in preB cells with BP9 treatment. (a) The gene expression profiles of BP9-treated WEHI-231 cells. WEHI-231 cells were treated with 0.01 mg/mL BP9 for 4 h, and the expressions of the differentially regulated genes were detected with microarray; the bar indicated that up- (red) and downregulated (green) genes were higher and lower 2-fold than that of control without BP9 treatment. (b) Gene expression validation. Seven differentially expressed genes obtained from WEHI-231 cells after BP9 treatment were validated with qRT-PCR. (c) The top 50 GO terms selected for finding the vital biological functions in preB cells with BP9 treatment. Blue, green, and orange bars indicated the biological processes, cellular component, and molecular functions among the top 50 GO terms, respectively. (d) Interaction network of top 50 GO terms in preB cells with BP9 treatment. The GO terms were generated by analyzing all the differentially expressed genes in WEHI-231 cells with BP9 treatment.

Article Snippet: The microarray experiment program was conducted by CapitalBio Technology Corporation (Beijing, China) as the previous methods [ ].

Techniques: Gene Expression, Microarray, Control, Biomarker Discovery, Quantitative RT-PCR, Generated